Fluorescence Filter

Specifications
Curve
Tutorial
Assembly
Feedback
Product Description
  • Fluorescence excitation filter, fluorescence emission filter, fluorescence dichroic mirror
  • BF33 substrate (excitation & emission), UVFS substrate (dichroic mirror)
  • Ø25 mm black mechanical retaining ring (excitation & emission), 36.0 mm x 25.0 mm (dichroic mirror)
LBTEK Fluorescence Filters are core specialized optical components for life science fluorescence imaging and optical detection equipment, including three product types: fluorescence excitation filters, fluorescence emission filters, and fluorescence dichroic mirrors, precisely compatible with a variety of mainstream biological fluorescent dyes such as DAPI, CFP, GFP, FITC, YFP, Cy3, TRITC, mCherry, Cy5, Cy5.5, and Cy7. The entire filter series offers advantages of high transmission, high blocking depth, and steep spectral edges, effectively suppressing stray light and channel crosstalk, significantly enhancing the signal-to-noise ratio and detection accuracy of fluorescence imaging. The fluorescence excitation and emission filters are mounted in a Ø25 mm black mechanical retaining ring, while the dichroic mirror uses the industry-standard bare size of 36.0 mm x 25.0 mm, perfectly compatible with mainstream optical systems such as 30 mm coaxial cage optical paths, fluorescence microscopes, filter wheels, and fluorescence filter cubes. In addition to standard products, LBTEK also provides custom solution for wavelength, bandwidth, and size. For custom requirements, please contact LBTEK technical support.
Attributes
Surface finish60/40
Surface Parallelism<3 arcmin
Surface Flatness (@633 nm)≤λ
Transmitted Wavefront Error (TWE) (@633 nm)≤λ/2
Fluorescence Filter Kit
  • Substrate: BF33&UVFS
  • Fluorescence Filter Kit
  • Fluorescence Excitation Filter & Fluorescence Emission Filter & Fluorescence Dichroic Filter

LBTEK Fluorescence Filter Kit is a standardized one-stop solution for fluorescence optical path, integrating excitation filter, emission filter, and dichroic mirror as a whole set, eliminating the need for user adjustment and matching. The product comprehensively covers mainstream biological fluorescence channels such as DAPI, GFP, FITC, Cy3, Cy5, and is suitable for life science imaging, in vitro diagnostic testing, and other scenarios. The product offers excellent optical performance, with passband peak transmission ≥90% and stop band cut-off depth OD≥6, effectively suppressing stray light and channel crosstalk, significantly improving imaging signal-to-noise ratio. Among them, the fluorescence excitation and emission filters are installed in a Ø25 mm black mechanical retaining ring, and the arrow (→) on the housing indicates the direction of beam propagation; the dichroic mirror has dimensions of 36.0 mm x 25.0 mm, and the tip of the side arrow (<) points to the surface coated with the filter film, facilitating installation and use. The fluorescence filter kit adopts industry-standard dimensions and can perfectly fit mainstream optical systems such as 30 mm coaxial cage optical path, fluorescence microscope, filter wheel, and fluorescence filter cube.

LBTEK Fluorescence Filter installed in microscope filter cube MRF1-MF2

Product Model
excitation bandwidth
emission bandwidth
fluorescent dye
File
Unit Price
Compare
Lead Time
MFFK-DAPI New365±40 nm460±50 nmDAPI
$428.83today
Add Cart
MFFK-CFP New435±20 nm480±30 nmCFP
$428.83today
Add Cart
MFFK-GFP New470±40 nm530±50 nmGFP
$428.83today
Add Cart
MFFK-FITC New480±35 nm535±50 nmFITC
$428.83today
Add Cart
MFFK-YFP New495±25 nm540±30 nmYFP
$428.83today
Add Cart
MFFK-CY3-TRITC New540±25 nm605±55 nmCY3、TRITC
$428.83today
Add Cart
MFFK-MCHERY New560±40 nm635±70 nmmChery
$428.83today
Add Cart
MFFK-CY5 New620±50 nm692±45 nmCY5
$428.83today
Add Cart
MFFK-CY5.5 New650±45 nm725±40 nmCY5.5
$428.83today
Add Cart
MFFK-CY7 New705±75 nm810±80 nmCY7
$428.83today
Add Cart
Fluorescence excitation filter
  • Base: BF33
  • Size: Ø25.0 mm x 3.5 mm, with mechanical snap ring
  • Angle of incidence: 0°±5°

LBTEK fluorescence excitation filters are manufactured using a low-autofluorescence BF33 high-borosilicate substrate, offering stable performance and no spectral shift, effectively eliminating stray light interference from the substrate at the source. The products employ a high-precision coating process that accurately matches the excitation bands of various fluorescent dyes, effectively filtering out ultraviolet, infrared, and ambient stray light from the light source, delivering a high-purity excitation light source and reducing non-specific signal interference. The filters feature a pass band transmission of ≥90% and an extinction depth of OD≥6, balancing high excitation efficiency with strong stray light suppression, making them suitable for weak fluorescence and high-precision microscopic detection applications. The filters are pre-installed in a Ø25.0 mm black anodized aluminum ring, with the light propagation direction indicated by an arrow (→) engraved on the housing for easy installation and use.

LBTEK Fluorescence Emission Filter Mounted on Coaxial Mounting Plate

Product Model
Center wavelength
Full Width at Half Maximum (FWHM)
fluorescent dye
File
Unit Price
Compare
Lead Time
MBF25-365-40 New365 nm±3 nm40 nm±3 nmDAPI
$167.35today
Add Cart
MBF25-435-20 New435 nm±3 nm20 nm±3 nmCFP
$167.35today
Add Cart
MBF25-470-40 New470 nm±3 nm40 nm±3 nmGFP
$167.35today
Add Cart
MBF25-480-35 New480 nm±3 nm35 nm±3 nmFITC
$167.35today
Add Cart
MBF25-495-25 New495 nm±3 nm25 nm±3 nmYFP
$167.35today
Add Cart
MBF25-540-25 New540 nm±3 nm25 nm±3 nmCY3、TRITC
$167.35today
Add Cart
MBF25-560-40 New560 nm±3 nm40 nm±3 nmmChery
$167.35today
Add Cart
MBF25-620-50 New620 nm±3 nm50 nm±3 nmCY5
$167.35today
Add Cart
MBF25-650-45 New650 nm±3 nm45 nm±3 nmCY5.5
$167.35today
Add Cart
MBF25-705-75 New705 nm±3 nm75 nm±3 nmCY7
$167.35today
Add Cart
Fluorescence emission filter
  • Base: BF33
  • Size: Ø25.0 mm x 3.5 mm, with mechanical snap ring
  • Angle of incidence: 0°±5°

LBTEK fluorescence emission filters utilize a low autofluorescence BF33 high-borosilicate substrate, offering stable performance with no spectral shift, and are suitable for various high-precision fluorescence imaging and detection applications. The products employ high-precision coating technology to precisely match the emission bands of various fluorescent dyes, efficiently purifying specific fluorescence signals while blocking residual excitation light, cell autofluorescence, and environmental stray light interference, resolving issues such as multi-channel crosstalk and blurred images. The pass band peak transmission is ≥90%, maximizing the retention of weak fluorescence signals, while the stop band blocking depth is OD≥6, achieving efficient stray light suppression and significantly enhancing image contrast and signal-to-noise ratio. The filters are pre-mounted in a Ø25.0 mm black anodized aluminum ring, with an arrow (→) engraved on the housing to indicate the light propagation direction for easy installation and use.

LBTEK Fluorescence Emission Filter Mounted on Coaxial Mounting Plate

Product Model
Center wavelength
Full Width at Half Maximum (FWHM)
fluorescent dye
File
Unit Price
Compare
Lead Time
MBF25-460-50 New460 nm±4 nm50 nm±4 nmDAPI
$167.35today
Add Cart
MBF25-480-30 New480 nm±3 nm30 nm±3 nmCFP
$167.35today
Add Cart
MBF25-530-50 New530 nm±3 nm50 nm±3 nmGFP
$167.35today
Add Cart
MBF25-535-50 New535 nm±3 nm50 nm±3 nmFITC
$167.35today
Add Cart
MBF25-540-30 New540 nm±3 nm30 nm±3 nmYFP
$167.35today
Add Cart
MBF25-605-55 New605 nm±4 nm55 nm±4 nmCY3、TRITC
$167.35today
Add Cart
MBF25-635-70 New635 nm±3 nm70 nm±3 nmmChery
$167.35today
Add Cart
MBF25-692-45 New692 nm±3 nm45 nm±3 nmCY5
$167.35today
Add Cart
MBF25-725-40 New725 nm±3 nm40 nm±3 nmCY5.5
$167.35today
Add Cart
MBF25-810-80 New810 nm±3 nm80 nm±3 nmCY7
$167.35today
Add Cart
Fluorescence Dichroic Mirror
  • Substrate: UVFS
  • Dimensions: 36.0 mm x 25.0 mm x 1.0 mm
  • Incident Angle: 45°±1.5°

LBTEK Fluorescence Dichroic Mirrors adopt a high-performance UV Fused Silica (UVFS) substrate, specifically designed for 45° oblique incidence optical paths. They feature high reflection in the short-wave range and high transmission in the long-wave range, enabling precise separation of optical path signals. The purified excitation light is reflected to excite the sample, while specific fluorescence signals are efficiently transmitted, ensuring a highly efficient and lossless optical path. The products have accurate spectral calibration, no crosstalk, and no stray reflections. Each model can be precisely matched with corresponding filter sets. Fluorescence Dichroic Mirrors come in a standard size of 36.0 mm x 25.0 mm x 1.0 mm, compatible with mainstream filter cubes, coaxial optical paths, and microscope modules. Both surfaces of the dichroic mirror are coated with a filter coating and an antireflection coating. The arrow (<) on the side points to the filter-coated surface. During use, light should enter from the filter-coated surface.

LBTEK fluorescence dichroic mirror is often installed in a 30 mm coaxial cube rectangular flat mount

Product Model
Reflection band
Transmission band
fluorescent dye
File
Unit Price
Compare
Lead Time
DMR-405LP New340-395 nm415-800 nmDAPI
$117.01today
Add Cart
DMR-456LP New350-450 nm462-800 nmCFP
$117.01today
Add Cart
DMR-500LP New350-495 nm510-800 nmGFP
$117.01today
Add Cart
DMR-506LP New350-500 nm512-800 nmFITC
$117.01today
Add Cart
DMR-520LP New450-510 nm525-800 nmYFP
$117.01today
Add Cart
DMR-562LP New480-555 nm570-850 nmCY3、TRITC
$117.01today
Add Cart
DMR-600LP New500-590 nm610-850 nmmChery
$117.01today
Add Cart
DMR-655LP New500-650 nm670-850 nmCY5
$117.01today
Add Cart
DMR-685LP New600-680 nm690-850 nmCY5.5
$117.01today
Add Cart
DMR-760LP New650-750 nm770-900 nmCY7
$117.01today
Add Cart