The LBTEK Optical Tweezers System Based on Vortex Waveplates mainly consists of a laser module, beam expansion module, polarization module, beam reduction module, dichroic mirror module, CMOS camera module, motion control module, and illumination module: 1) The laser output is expanded in beam diameter after passing through the beam expansion system. 2) The expanded beam first passes through a polarizer, converting the entire beam into linearly polarized light. It then passes through a quarter-wave plate at a specific angle, transforming the beam into circularly polarized light. Finally, after passing through the vortex waveplate, the emitted beam becomes a vortex beam with a specific topological charge. Rotating the quarter-wave plate changes the sign of the topological charge of the vortex beam. 3) The beam reduction system shrinks the beam for easier entry into the objective lens. 3) A high-power oil immersion objective lens is selected to achieve a finer tweezer tip and greater trapping force. 4) The sample is placed on an XYZ three-axis translation stage, enabling displacement along the X, Y, and Z axes. 5) Light from the white LED illumination source is shaped and then directed onto the sample under observation. 6) The illumination light carrying laser-trapped sample information passes through the objective lens, transmits through the dichroic mirror, and is reflected by a mirror before being incident on the CMOS camera for imaging. 7) Considering that the dichroic mirror cannot completely isolate the laser, some of the laser light reflected from the sample will partially transmit through the dichroic mirror and reach the CMOS, affecting imaging quality. A filter is added in front of the CMOS to further block the laser beam. An achromatic lens is also included to adjust the field of view and enhance the brightness of the light incident on the CMOS.