Basic Edition Single-Beam Optical Tweezers Systems

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Product Description
  • The laser optical trapping force can be freely used to capture particles and manipulate their movement
  • The XYZ three-axis translation stage allows convenient displacement adjustment to achieve relative motion between particles and the laser
  • By connecting a CMOS camera to a computer, the laser-manipulated particle motion can be displayed in real-time
  • It can be installed on a 30 cm × 45 cm optical breadboard for easy overall movement without repeated adjustments
  • A complete set of particle preparation tools, system installation tools, and detailed product manuals are provided for convenient user setup and operation
The basic version of LBTEK's Single-Beam Optical Tweezers System mainly consists of a laser module, dichroic mirror module, CMOS camera module, motion control module, and illumination module: 1) The laser outputs a beam that is incident on the dichroic mirror; 2) The cutoff wavelength of the dichroic mirror is selected to reflect the laser while allowing visible light to pass through. Generally, a short-pass dichroic mirror is chosen, with its cutoff wavelength shorter than the laser wavelength; 3) A high-power oil immersion objective is selected to achieve a finer trapping spot and greater trapping force; 4) The sample is placed on an XYZ three-axis displacement stage, enabling movement along the X, Y, and Z axes; 5) The light emitted by the white LED illumination source is shaped and then directed onto the sample under observation; 6) The illumination light carrying the laser-trapped sample information passes through the objective, transmits through the dichroic mirror, and is reflected by a mirror before being incident on the CMOS camera for imaging; 7) Considering that the dichroic mirror cannot completely block the laser, some of the laser reflected from the sample will partially transmit through the dichroic mirror and reach the CMOS, affecting imaging quality. A filter is added in front of the CMOS to further eliminate the laser beam, and an achromatic lens is used to adjust the field of view while enhancing the brightness of the light incident on the CMOS. LBTEK will soon offer an upgraded standard single-beam optical tweezers system. In addition to all the functions of the basic optical tweezers system, the standard system is equipped with interfaces for connecting expansion modules. It can easily integrate a vortex beam module to drive particle rotation or a fluorescence module for observing the structure and properties of biological cells. For more details about the system, please contact LBTEK technical support.
Attribute
light source665 nm
White LEDMaximum output optical power 3 W, with power adapter
Objective lens100x, oil immersion, NA 1.25, RMS thread
Sample4 μm silica spheres, 4 μm polystyrene microspheres
Breadboard and handleBreadboard 300×450 mm, double handle
CMOS cameraGigE interface/2592×1944/2.2 μm×2.2 μm
Basic Optical Tweezer Systems
  • Laser wavelength: 685nm
  • System size: Mounted on a 30cm×45cm portable optical breadboard
  • Particles and size: 4μm silica microspheres
  • 65×65mm 3-axis side-drive translation stage, 13mm travel
  • White LED illumination, CMOS monochrome camera
The LBTEK Basic Edition Single Beam Optical Tweezer System is shipped as component parts, including a complete set of particle preparation tools, system installation tools, CMOS camera installation software, and detailed product manuals. Users can install and use the system themselves by following the installation steps provided in our product manual. Of course, they also have the option to freely choose on-site installation services by our engineers.

Experimental Results Display

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Description
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Lead Time
OTB-V1 Used to capture and manipulate particle movement, displaying the motion of particles in real-time via a CMOS camera.$4422.976 weeks
Experimental Sample Preparation Kit
  • Microscope slides, 24 mm × 50 mm (L × W), thickness 0.13-0.16 mm
  • Cover slips, 20 mm × 20 mm (L × W), thickness 0.13-0.16 mm
  • Silica microspheres in deionized water, Ø4.0 µm, 10mL
  • Polystyrene microspheres in deionized water, Ø4.0 µm, 10mL
  • Micropipette with 200 µL capacity
  • 10 double-sided adhesive sheets, 8 wells/sheet
  • 2 bottles of deionized water, 15mL/bottle
The SP1-KIT is a sample preparation tool suitable for the OTB-V1 basic version optical tweezers and biological experiments. The prepared samples have a long duration and can be used for multiple experiments. This kit includes the above components.
Sample Preparation Kit
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Lead Time
SP1-KIT Applicable to OTB-V1 Basic Edition Optical Tweezer and biological experiment sample preparation tools$577.482 weeks
Fixed Microscope Slide Holders
  • Compatible slides wider than 44 mm
  • Compatible petri dish diameter: 37.1 mm~41.4 mm
  • Mounted on a translation mount with M6 tapped holes spaced 50 mm apart

The LBTEK Fixed Microscope Slide Holder is designed for petri dishes and microscope slides on the Z-axis of LBTEK's three-axis translation platform, enabling integration into customized microscope setups such as optical tweezers. Two sets of mounting holes provide versatile applications: one set features four M4 countersunk holes with a 12.5 mm spacing, while the other has two M6 countersunk slots with a 50 mm spacing, ensuring compatibility with various components. The spring clips can be rotated for easy replacement of glass slides and petri dishes. The OT-SH1 clamp features a central 25.4 mm clear aperture for light transmission. The OT-SH35 offers a larger 35 mm clear aperture, accommodating not only microscope slides but also test targets, large-diameter objectives, and other rectangular optical components with widths exceeding 44.0 mm.

Fixed microscope slide holders are commonly used to hold slides, etc.

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Clear aperture
Petri dish well depth
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Lead Time
OT-SH35 35 mm3 mm$96.366 weeks
OT-SH1 25.4 mm5 mm$99.235 weeks
Right- Angle Mounting Plate
  • Right-angle mounting plate
  • M4 and M6 tapped holes with 25mm spacing on all mounting surfaces
  • Three-sided M4 tapped mounting hole configuration on PB-5 for three-directional installation
  • Compatible with metric or imperial breadboards, posts, and brackets

The right-angle mounting plate from LBTEK measures 65 mm × 65 mm × 75 mm and is comprised of two PB-5. The M6 and M4 tapped hole arrays enable multi-directional component mounting. Purchase of the LBTEK RAB1 comes with four M6×8mm socket head cap screws (with flat washers).

The right-angle mounting plate is installed on a three-axis translation stage.

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Overall Dimensions
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Lead Time
RAB1 65 mm×65 mm×75mm$54.264 weeks
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