The LBTEK imaging spacer is made of adhesive tape with adhesive on both sides. By peeling off the protective film and pressing it onto a coverslip or slide, it forms a sealed chamber that supports free-floating samples, allowing for the resolution of fine internal structures. The shape of the spacer's wells remains stable without compression, and multiple spacers can be stacked to customize chambers to any desired depth. It is suitable for applications such as optical tweezers and single-molecule spectroscopy techniques.